Growth of Venezuelan equine encephalomyelitis virus in human diploid cell strain WI-38.
- 1 January 1970
- journal article
- Vol. 19 (1) , 196-8
Abstract
We demonstrated that Venezuelan equine encephalomyelitis (VEE) virus replicated in and adapted rapidly to human diploid cell strain WI-38. Peak titers of approximately 10(9.8) mouse intracerebral 50% lethal doses were obtained at low passage levels in Eagles basal medium supplemented with calf serum. VEE virus replicated poorly in serum-free medium. Propagation of VEE virus was accompanied by the production of hemagglutinin and cytopathogenic effects.This publication has 9 references indexed in Scilit:
- The limited in vitro lifetime of human diploid cell strainsPublished by Elsevier ,2004
- Nonviable Venezuelan equine encephalomyelitis hemagglutinin prepared from tissue cultures by gamma radiation.1969
- GROWTH OF RIFT VALLEY FEVER VIRUS IN HUMAN DIPLOID (WI-38) CELLSAmerican Journal of Veterinary Research, 1968
- Successful Immunization of Primates with Rabies Vaccine Prepared in Human Diploid Cell Strain WI-38.Experimental Biology and Medicine, 1965
- THE IN VITRO GROWTH OF RUBELLA VIRUS IN HUMAN EMBRYO CELLS1American Journal of Epidemiology, 1965
- Application of a Microtechnique to Viral Serological InvestigationsThe Journal of Immunology, 1962
- THE GROWTH OF VENEZUELAN EQUINE ENCEPHALO-MYELITIS VIRUS IN VARIOUS TISSUE CULTURES1American Journal of Epidemiology, 1959
- Nutrition Needs of Mammalian Cells in Tissue CultureScience, 1955
- Propagation of Venezuelan equine encephalomyelitis virus in cultures of human malignant epithelial cells, strain HeLa.1955