GASTRIC-EMPTYING OF A SOLID-LIQUID MEAL IN HEALTHY-SUBJECTS - VALIDITY OF CHICKEN LIVER LABELING (TC-99M) BY MULTIPUNCTURE TECHNIQUE

  • 1 January 1985
    • journal article
    • research article
    • Vol. 9  (4) , 342-347
Abstract
For gastric emptying studies of a solid-liquid meal by the scintigraphic method, a valid isotope labeling method for each phase of the meal must be obtained. A simple chicken liver labeling method was validated in normal subjects by multipuncture technique with 99mTc. Labeling according to Meyer''s method was chosen as a reference. A study of the quality of liquid phase labeling by 111In was done. The labeling process quality for each phase of the meal was assessed in vitro, after incubation of the meal with human gastric juice (n = 12); in vivo, after meal ingestion and sequential collection of gastric contents by aspiration (n = 4). In 8 healthy volunteers, gastric emptying curves of the solid and liquid phases of the meal were determined scintigraphically and compared. For the solid phase a good specificity of the marker, which was assessed in vitro and in vivo, after liver labeling with multipuncture technique (89/100 and 92/100 after 180 min, respectively) was shown. The liquid phase showed a good specificity of the marker in vitro and a poor specificity in vivo (82/100 and 27/100 after 180 min, respectively); Similar half-gastric emptying times and cumulative percentages for the solid and liquid phases with both liver labeling methods were shown. The multipuncture technique for chicken liver labeling may be used for gastric emptying studies in humans.