Influence of Sodium‐Independent γ‐Aminobutyric Acid Binding on the Assay of Sodium‐Dependent γ‐Aminobutyric Acid Binding in a Membrane Preparation of Rat Brain
- 1 January 1981
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 36 (1) , 313-316
- https://doi.org/10.1111/j.1471-4159.1981.tb02413.x
Abstract
A large amount of [3H]GABA was bound to crude synaptic membrane fractions of rat, by a Na-independent process in a medium containing 100 .mu.M [3H]GABA used for assaying the GABA uptake site. This [3H]-GABA binding was different from receptor binding of GABA. This Na-independent [3H]GABA binding scarcely occurred in the presence of a physiological concentration of NaCl. Na-independent GABA binding had a negligible influence on Na-dependent GABA binding.Keywords
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