Preparation of ligand-free human serum for radioimmunoassay by adsorption on activated charcoal.
- 1 February 1978
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 24 (2) , 362-364
- https://doi.org/10.1093/clinchem/24.2.362
Abstract
I describe a convenient method for stripping hormones and other ligands from human serum. Formation of a serum/charcoal colloid is avoided by incubating serum on a limited surface area with activated charcoal, in a lateral oscillating shaker. Particle-free serum of negligible turbidity is obtained without ultracentrifugation by ejecting the centrifugate through a cellulose ester membrane. By this technique, more than 80% of thyrotropin, triiodothyronine, thyroxine, cortisol, insulin, digoxin, folate, estriol, or an ether derivative of testosterone is removed from serum. About 70% of follitropin and somatotropin are removed, as well as half the choriogonadotropin. Serum protein remains intact throughout the adsorption process.This publication has 1 reference indexed in Scilit: