DETERMINATION OF URINARY METABOLITES HYDROXYINDOLE-ACETIC ACID, VANILLYL MANDELIC-ACID AND HOMOVANILLIC-ACID BY MEANS OF LIPOPHILIC GEL CHROMATOGRAPHY AND GAS-CHROMATOGRAPHY

  • 1 January 1977
    • journal article
    • research article
    • Vol. 15  (2) , 81-87
Abstract
A specific and practicable method is described for the quantitative determination of urinary phenol- and indole-carboxylic acids. High specificity is achieved by a preliminary separation of the free acids (extracted from the urine sample) with the aid of organophilic gel chromatography on Sephadex LH 20, followed by gas chromatographic analysis of the silyl derivatives of the acids. The organophilic gel chromatography of the free acids shows a high recovery rate in the micro- and submicrogram range. The difficulties encountered in other techniques in the derivatization and gas chromatographic separation of the individual components are avoided by using the preliminary separation, and by using N-methyl-N-trimethylsilyl-trifluoroacetamide for the derivatization. Use of this preparation technique with a direct read-out gas chromatograph with automatic sample introduction, gives high accuracy and precision, and a facility for the determination of a wide range of aromatic acids in human urine.

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