CHANGES IN ACTIVITIES OF CREATINE KINASE, ARGININE KINASE AND THEIR MULTIENZYME FORMS DURING EMBRYONIC AND LARVAL DEVELOPMENT OF SEA URCHINS

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Abstract
The presence and the changes of CPK [ATP-creatine-N-phosphotransferase EC 2.7.3.2] and APK [ATP-arginine-N-phosphotransferase EC 2.7.3.3] were studied during larval development through metamorphozing of Hemicentrotus pulcherrimus, Anthocidaris crassispina and Pseudocentrotus depressus. While no CPK activity was found in unfertilized eggs and embryos of early developmental stages, APK was quite active throughout these stages. At the late 8-armed pluteus stage just prior to metamorphosis, CPK first became active. Electrophoretically this CPK was identical with 1 of 3 CPK forms of sperm, tube feet and esophagus but not with 2 CPK forms of lantern muscle. APK in the unfertilized eggs and early embryos was electrophoretically separated into 2 distinct molecular forms, 1 of which disappeared during the late larval stages. The persisting form of larval APK was identical with a single APK form present in the adult muscular tissues.