Formation of Catechol Oxidase in Seed Coats ofPisum elatiusduring Seed Maturation

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Abstract
The catechol oxidase present in the seed coat of Pisum elatius at various stages of seed maturation was partially characterized. The pH dependence and response to inhibitors and stimulators of the enzyme was determined. Inhibitors of protein synthesis and enzyme activity were introduced into the developing seeds via the pod. During the early stages of seed maturation, the increased enzyme activity was probably due to de novo synthesis while the increase in the later stages of maturation can best be accounted for on the basis of activation of pre-existing enzyme, perhaps due to conformational changes in the enzyme. The evidence for this view is discussed.

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