Cloning and characterization of the Streptomyces peucetius dnmZUV genes encoding three enzymes required for biosynthesis of the daunorubicin precursor thymidine diphospho-L-daunosamine
- 1 July 1997
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 179 (13) , 4446-4450
- https://doi.org/10.1128/jb.179.13.4446-4450.1997
Abstract
Characterization of the dnmZ, dnmU, and dnmV genes from the daunorubicin-producer Streptomyces peucetius by DNA sequence analysis indicated that these genes encode a protein of unknown function plus a putative thymidine diphospho-4-keto-6-deoxyglucose-3(5)-epimerase and thymidine diphospho-4-ketodeoxyhexulose reductase, respectively. Inactivation of each of the three genes by gene disruption and replacement in the wild-type strain demonstrated that all of them are required for daunosamine biosynthesis.Keywords
This publication has 30 references indexed in Scilit:
- The dnrM gene in Streptomyces peucetius contains a naturally occurring frameshift mutation that is suppressed by another locus outside of the daunorubicin-production gene clusterMicrobiology, 1996
- Molecular and biochemical analyses of fatty acid transport, metabolism, and gene regulation in Escherichia coliBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1994
- Characterization of the dTDP‐rhamnose biosynthetic genes encoded in the rfb iocus of Shigella flexneriMolecular Microbiology, 1994
- Biosynthesis of 3,6-dideoxyhexoses: new mechanistic reflections upon 2,6-dideoxy, 4,6-dideoxy, and amino sugar constructionJournal of the American Chemical Society, 1993
- Molecular basis of 3,6-dideoxyhexose biosynthesis: elucidation of CDP-ascarylose biosynthetic genes and their relationship to other 3,6-dideoxyhexose pathwaysJournal of the American Chemical Society, 1993
- Purification and characterization of TDP-D-glucose 4,6-dehydratase from anthracycline-producing streptomycetesJournal of General Microbiology, 1992
- Basic local alignment search toolJournal of Molecular Biology, 1990
- Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coliGene, 1988
- Prediction of the occurrence of the ADP-binding βαβ-fold in proteins, using an amino acid sequence fingerprintJournal of Molecular Biology, 1986
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985