Evaluation of Erysiphe graminis f sp tritici field isolates for resistance to strobilurin fungicides with different SNP detection systems
- 11 February 2003
- journal article
- research article
- Published by Wiley in Pest Management Science
- Vol. 59 (3) , 310-314
- https://doi.org/10.1002/ps.639
Abstract
A single nucleotide polymorphism (snp) in the cytochrome b gene confers resistance to strobilurin fungicides in Erysiphe graminis DC f sp tritici Marchal. On the basis of this point mutation three different types of molecular markers have been developed. Cleaved amplified polymorphic sequences and allele‐specific PCR were used to score resistant and sensitive isolates from specifically selected regional populations across Europe. The results of molecular tests were in total agreement with the resistance phenotypes revealed by in vivo tests. Serial dilutions of mixed samples (resistant/sensitive) delimited the detection for strobilurin‐resistant alleles to a range of 10–50% for both marker classes. Due to these detection limits no mixture of mitochondria within individual isolates was found. Denaturing high performance chromatography was used to increase the detection sensitivity for the mutant allele. Although the detection limit was lowered to 5–10%, there was no evidence for the existence of mixed mitochondrial genotypes. © 2003 Society of Chemical IndustryKeywords
This publication has 11 references indexed in Scilit:
- Isogamous, hermaphroditic inheritance of mitochondrion-encoded resistance to Qo inhibitor fungicides in Blumeria graminis f. sp. triticiFungal Genetics and Biology, 2002
- Characterizing resistance risk of Erysiphe graminis f.sp. tritici to strobilurinsPublished by Elsevier ,2001
- Point Mutation in Cytochrome b Gene Conferring Resistance to Strobilurin Fungicides in Erysiphe graminis f. sp. tritici Field IsolatesPesticide Biochemistry and Physiology, 2000
- The Molecular Basis for the Natural Resistance of the Cytochrome bc1 Complex from Strobilurin‐Producing Basidiomycetes to Center QP InhibitorsEuropean Journal of Biochemistry, 1996
- Mitochondrial genetics of Chlamydomonas reinhardtii: resistance mutations marking the cytochrome b gene.Genetics, 1991
- Mucidin and strobilurin A are identical and inhibit electron transfer in the cytochrome bc1 complex of the mitochondrial respiratory chain at the same site as myxothiazolBiochemistry, 1986
- Oudemansin, strobilurin A, strobilurin B and myxothiazol: new inhibitors of the bc1 segment of the respiratory chain with an E‐β‐methoxyacrylate system as common structural elementFEBS Letters, 1981
- A High Throughput Jet Trap for Collecting Mildew Spores on Living LeavesJournal of Phytopathology, 1979
- The strobilurins - New antifungal antibiotics from the basidiomycete Strobilurus tenacellus.The Journal of Antibiotics, 1977
- Population GeneticsPublished by Springer Nature ,1976