Determination of Propionyl-CoA Dehydrogenase Activity inSalmonella TyphimuriumLT-2 Cells by HPLC

Abstract
A reverse-phase high-performance liquid chro matographic system was developed for the detection of propionyl-CoA dehidrogenase activity in the catabolic pathway of propionate from bacterial extracts, in the presence of some interferent activities. The method is also useful for the separation and identification of free CoASH and some of its derivatives (acetyl-CoA, propionyl-CoA and acrylyl-CoA).