Use of Thin, Flexible Plastic Coverslips for Microscopy, Microcompression, and Counting of Aerobic Microorganisms

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Abstract
A new method of microscopic slide preparation characterized by low toxicity and high gaseous exchange is described using nonwettable thin plastic film (Handiwrap) as the coverslip and plastic slides. The preparation can be used with oil immersion (100 .times.) lens with some modifications. Layers of high vacuum silicone grease are used to surround the film and reduce evaporation, creating a very long-lived, well-aerated preparation. Removing excess H2O pulls the plastic film down, creating a controllable microcompressor effect. Such thin preparations have excellent optical qualities. Various applications are discussed.

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