QUALITATIVE STUDIES OF SOIL MICROORGANISMS: XI. FURTHER OBSERVATIONS ON THE NUTRITIONAL CLASSIFICATION OF BACTERIA
- 1 July 1953
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Botany
- Vol. 31 (4) , 438-447
- https://doi.org/10.1139/b53-035
Abstract
A review of the method developed in this laboratory in 1943 for the nutritional classification of soil bacteria has suggested slight amendments in certain differential media: (1) the substitution of vitamin-free casamino acids for a combination of amino acids, and (2) the addition of vitamin B12 to the growth factor media. In a comparative study with a newly proposed scheme of classification, the more selective plating medium advocated was found to be less suitable for the isolation of soil bacteria than the nonselective soil extract agar in the original method. Furthermore, the replacement of potassium nitrate with diammonium phosphate as source of inorganic nitrogen in the basal medium failed to cause any significant change in the nutritional grouping. Results from the nutritional classification of some 600 isolates by the two methods showed that the new procedure represents only a slight modification of the original system.Keywords
This publication has 1 reference indexed in Scilit:
- QUALITATIVE STUDIES OF SOIL MICRO-ORGANISMS: IV. THE RHIZOSPHERE IN RELATION TO THE NUTRITIVE REQUIREMENTS OF SOIL BACTERIACanadian Journal of Research, 1940