Isolation and characterization of yeast mutants auxotrophic for 2′-deoxythymidine 5′-monophosphate
- 1 January 1979
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 168 (2) , 141-151
- https://doi.org/10.1007/bf00431440
Abstract
Mutant strains of Saccharomyces cerevisiae auxotrophic for deoxythymidine monophosphate (dTMP) were isolated and characterized. Two distinct classes of auxotrophs were obtained. One class had a simple requirement for dTMP and was analogous to thymine-requiring bacteria. The second class required dTMP, adenine, histidine and methionine and this complex nutritional phenotype was due to defects in folate metabolism. The dTMP-dependent growth of respiratory-competent grande auxotrophs was found to be markedly affected by media composition and carbon source. In the absence of dTMP thymineless death occurred in both mutant classes.This publication has 43 references indexed in Scilit:
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