Degradation of Adsorbed Transforming DNA by Haemophilus influenzae

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Abstract
SUMMARY Radioactively labelled transforming DNA is rapidly and irreversibly adsorbed by competent Haemophilus influenzae bacteria. Upon incubation, about 20 yo is degraded within 15 min. to acid-soluble products. Thereafter there is little change. Degradation products were identified by Dowex column chromatography as inorganic phosphate, nucleosides, small and large oligo- nucleotides. Mononucleotides were not observed. These products were always found in the medium while little, if any, was observed in the cells. It is concluded that the degradation process may take place outside the cells. It involves at least an endonuclease and a phosphatase.
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