A method of separating antibodies from serum proteins. Investigations on a protein-free antibody

Abstract
Working with diphtheria antitoxin and antityphoid serum, Frankel and Olitzki (1930) demonstrated that it is possible to separate the active antibodies from the serum proteins. The solutions of antibodies obtained were free from protein as indicated by sensitive chemical reactions and confirmed biologically by sensitisation tests. It was found (Olitzki and Frankel, 1931) impossible to sensitise guinea pigs by injecting such solutions of antibodies both against the solutions themselves as well as against the original untreated sera. The present paper contains (1) a complete description of a method of separating antibodies from serum proteins (including improvements made subsequent to previous publications), (2) the results of purification and concentration of antibody and chemical investigations on the protein-free flagellar typhoid agglutinin, particularly the action of proteolytic enzymes.

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