Hormonal control of morphogenesis in leaf segments ofCentaurium erythraea

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Abstract
Transversally cut leaf segments ofCentaurium erythraea were cultivated on MS medium. Effects of segment polarity, IAA and sucrose concentrations, light and medium volume on morphogenesis were studied. Shoots generally formed at lower (1.3 × 10-6 mol 1-1) IAA concentrations than roots and callus (1.1 × 10-5−3.4 × 10-5 mol 1-1). Leaf polarity strongly modified the effect of IAA concentration, shifting organogenesis at the segment base toward decreased IAA concentrations as compared with segment apex. Light, sucrose concentration above 3 % and high medium volume changed IAA dependence of morphogenesis in various ways and generally suppressed segment polarity.