Effect of Temperature on Natural Mutation in Escherichia coli

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Abstract
The mutation rate from histidine auxotrophy to prototrophy in Escherichia coli was measured during growth and in the stationary phase at different temperatures. During growth the rate of mutation/mutable unit/hr. had the same temperature coefficient as growth rate. Therefore the rate of mutation/mutable unit/generation was the same at all temperatures. The temperature coefficient of the rate of mutation during the stationary phase may be the same as that during growth, indicating that although mutation is slower in this phase it may involve the same process as during growth.

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