Reconstitution of T Cell Receptor ζ-Mediated Calcium Mobilization in Nonlymphoid Cells
- 13 August 1993
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 261 (5123) , 915-918
- https://doi.org/10.1126/science.8346442
Abstract
T cell antigen receptor (TCR) activation involves interactions between receptor subunits and nonreceptor protein tyrosine kinases (PTKs). Early steps in signaling through the ζ chain of the TCR were examined in transfected COS-1 cells. Coexpression of the PTK p59 fynT , but not p56 lck , with ζ or with a homodimeric TCRβ-ζ fusion protein produced tyrosine phosphorylation of both ζ and phospholipase C (PLC)-γ1, as well as calcium ion mobilization in response to receptor cross-linking. CD45 coexpression enhanced these effects. No requirement for the PTKZAP-70 was observed. Thus, p59 fynT may link ζ directly to the PLC-γ1 activation pathway.Keywords
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