The Comparative Virogenesis of Three Serotypes of Bluetongue Virus in Culicoides Variipennis (Diptera: Ceratopogonidae)1

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Abstract
Studies were conducted to investigate the comparative ability of 3 serotypes of bluetongue virus (BTV) to infect and replicate in Culicoides variipennis. The virogenesis or mode of development of each serotype of BTV in the fly was determined using a direct immunofluorescence technique. BTV serotypes 10, 11, and 17 were each found to be capable of infecting C. variipennis. Susceptibility of the flies to the different serotypes was comparable, and each virus serotype replicated in individual flies to an average titer of 3.2 ± 0.3 log10 TCID50. In addition, each serotype exhibited the same general virogenesis. Antigen was first detected in midgut cells and subsequently in secondary target tissues, including hindgut, fat body, salivary gland, thoracic muscle, and ovarian tissues excluding follicles and eggs. Nerve tissue infrequently contained detectable antigen. The 3 serotypes tested were similar in their respective abilities to infect and replicate in C. variipennis.

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