A Rapid Chromatographic Procedure for the Isolation and Quantitation of 1-(2-Aminoethyl)-3-(2,6-dichlorophenyl) thiourea from Dietary Admix Followed by HPLC
- 1 November 1980
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Chromatographic Science
- Vol. 18 (11) , 622-625
- https://doi.org/10.1093/chromsci/18.11.622
Abstract
An analytical procedure is described for the determination of 1-(2-aminoethyl)-3-(2,6-dichiorophenyl)thiourea in dietary admix. Because of the complex nature of the carrier matrix, sample cleanup was required prior to HPLC separation. Sample cleanup was achieved using a cation-exchange microcolumn which yielded highly reproducible results. HPLC separation was accomplished using a 10 μ C18 column, monitoring the column effluent in the ultraviolet region at 254 nm. After sample cleanup, 1-(2-aminoethyl)-3-(2,6-dichlorophenyl) thiourea could be detected at levels as low as 3 ppm in dietary admix.This publication has 0 references indexed in Scilit: