In Vitro Micrografting of Apple Shoot Tips1

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Abstract
Micrografting of apple (Malus domestica Borkh.) was achieved by placing a 0.1 to 0.2 mm scion explant derived either from field-grown or in vitro-grown shoot apices on the hypocotyl of a decapitated 15 day-old seedling. Under asceptic conditons, the scion and hypocotyl unite to form a complete plant which produces 4 to 6 leaves after 6 weeks. These plants were transplanted to sterile vermiculite and conditioned to ambient humidity for 1 week and then transplanted to soil. This technique was used to obtain virus-free plants from virus-infected plants.

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