SUBUNIT STRUCTURE AND KINETIC PROPERTIES OF 4‐AMINOBUTYRATE‐2‐KETOGLUTARATE TRANSAMINASE PURIFIED FROM MOUSE BRAIN

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Abstract
Abstract— The effects of divalent metal ions, sulfhydryl reagents, carbonyl trapping reagents, substrate analogs, and organic solvents on purified mouse brain 4‐aminobutyrate‐2‐ketoglutarate transaminase (EC 2.6.1.19) and the subunit structure of this enzyme were studied. Of the metal ions tested, Hg2+ was found to be the most potent inhibitor inhibiting the enzyme 50 percent at a concentration of 0‐7 μM. The order of decreasing inhibitory potency for the divalent metal ions was: Hg2+± Cd2+± Zn2+± Cu2+± Co2+± Ba2+± Sr2+± Ni2+± Mn2+± Ca2+± Mg2+. p‐Chloromercuribenzoale was the most potent inhibitor among the sulfhydryl reagents tested inhibiting the enzyme to the extent of 50 per cent at 0‐5 μM 3‐Mercaptopropionic acid was found to be a competitive inhibitor for GABA and non‐competitive for 2‐ketoglutarate. The Ki, value was estimated to be 13 μM. Aminooxyacetic acid was the most potent inhibitor of the carbonyl trapping agents with a K, value of 0‐06 μM. being competitive with GABA and non‐competitive with 2‐ketoglutarate. Hydroxylamine and hydrazine were the next most potent compounds in this group. Of a series of substrate analogs and metabolites tested, only acetic acid, propionic acid, butyric acid, glutamic acid, adipic acid, pimelic acid and 2‐ketoadipic acid inhibited the enzyme to a significant extent. Dioxan inhibited the enzyme 50 per cent at a concentration of 5 per cent (v/v) whereas methanol and ethanol only inhibited 5‐10 per cent at 10 per cent (v/v) concentration.A spectrum of the native enzyme at pH 7‐2 showed maxima at 278 nm. 330 nm and 411 nm. Treatment of the enzyme with aminooxyacetic acid or 3‐mercaptopropionic acid caused the maximum at 411 nm to disappear.Sodium dodecyl sulfate polyacrylamide gel electrophoresis of the enzyme revealed two protein bands. The molecular weights of these two subunits were determined to be 53.000 and 58,000, respectively.