Comparison of DNA extraction and amplification from ancient human bone and mummified soft tissue
- 1 May 1994
- journal article
- research article
- Published by Springer Nature in International journal of legal medicine
- Vol. 107 (3) , 152-155
- https://doi.org/10.1007/bf01225603
Abstract
South american precolumbian male mummies were employed as source material for a comparative investigation of bone and soft tissues by DNA analysis. The suitability of the DNA extracts from both sources was tested and evaluated by their effectiveness as target DNA in PCR amplifications. The results suggest that skeletal material should be given preference over soft tissues for PCR analysis if the material is severely degraded. This seems to be independent of the specific anatomical origin of the samples. Für eine vergleichende Untersuchung von Knochen und Weichgewebe als Quellenmaterial für DNA-Analysen wurden südamerikanische, präkolumbische, männliche Mumien verwendet. Die Eignung der DNA-Extrakte beider Quellen wurde über ihre Effektivität als Ziel-DNA in PCR-Amplifikationen bewertet. Die Ergebnisse zeigen, daß bei Verwendung stark degradierten Materials Knochen den Weichgeweben für PCR-Analysen vorzuziehen sind. Dies scheint unabhängig von der spezifischen anatomischen Herkunft der Proben zu sein.Keywords
This publication has 14 references indexed in Scilit:
- Identification of the remains of the Romanov family by DNA analysisNature Genetics, 1994
- Y-Chromosomal DNA from Ancient BonesPublished by Springer Nature ,1994
- Specificity, efficiency, and fidelity of PCR.Genome Research, 1993
- Protocol for ultraviolet irradiation of surfaces to reduce PCR contamination.Genome Research, 1993
- Identification of the skeletal remains of josef mengele by DNA analysisForensic Science International, 1992
- Improved efficiency in amplification of ancient DNA and its sequence analysisThe Science of Nature, 1992
- Typing of Deoxyribonucleic Acid (DNA) Extracted from Compact Bone from Human RemainsJournal of Forensic Sciences, 1991
- Identification of the skeletal remains of a murder victim by DNA analysisNature, 1991
- Recovery and analysis of human genetic material from mummified tissue and boneJournal of Archaeological Science, 1990
- An Improved Method for Prenatal Diagnosis of Genetic Diseases by Analysis of Amplified DNA SequencesNew England Journal of Medicine, 1987