Early High Dose Antioxidant Vitamins Do Not Prevent Bronchopulmonary Dysplasia in Premature Baboons Exposed to Prolonged Hyperosia: APilot Study

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Abstract
The antioxidant vitamins ascorbic acid (AA) and α-tocopherol(α-TP) effectively inhibit oxygen free radical-induced lipid peroxidation. Using a premature baboon model of hyperoxiainduced bronchopulmonary dysplasia (BPD), we measured concentrations of AA,α-TP, and conjugated dienes (CD, marker of lipid peroxidation) in four animals (hyperoxic antioxidant group) receiving high dose antioxidant vitamin supplementation (AA, 100 mg·kg·-1·d-1;α-TP; 20 mg·kg·-1·d-1) and one animal receiving standard dose antioxidant vitamin supplementation (AA, 10 mg·kg·-1·d-1; α-TP, 1 mg·kg·-1·d-1). Respiratory and histopathologic data were compared with data from 10 historical animals exposed to hyperoxia (hyperoxic control group) and 11 historical animals treated as required with oxygen (normoxic control group) who had received standard dose antioxidant vitamin supplementation. Compared with standard dose antioxidant vitamin supplementation, high dose antioxidant vitamin supplementation, effectively raised AA concentrations in plasma (37 ± 22 μmol/L and 395 ± 216 μmol/L, respectively) and tracheal aspirates (62 ± 35 μmol/L and 286 ± 205 μmol/L, respectively), and α-TP concentrations in plasma (10.1 ± 2.5μmol/L and 24.6 ± 17.5 μmol/L, respectively). However, there was no apparent effect on tracheal aspirate CD concentrations (482 ± 333μmol/L and 1050 ± 1111 μmol/L, respectively), and respiratory parameters in the hyperoxic antioxidant group were comparable to those of the hyperoxic control group but significantly worse than in the normoxic control group. Finally, no protective effect of high dose antioxidant vitamin supplementation was noted at the histopathologic level.