Effect of horse antibody to rat alpha‐fetoprotein upon the growth of AH‐66 in donryu rats

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Abstract
Whether the i.p. administration of horse antibody to rat .alpha.-fetoprotein (HabRatAFP) would inhibit or prevent the growth of AH-66 tumor cells inoculated into Donryu male rats was studied. In animals inoculated with tumor s.c. there was 100% tumor growth in uninjected control animals and the administration of HabRatAFP prevented tumor growth in 7/24 (29%) of the animals. When animals were inoculated with tumor i.p., the inhibition of tumor growth by HabRatAFP only occurred at tumor-cell doses of 50,000, 10,000 and 5000. In these 3 groups, 15/35 (43%) of those animals treated with HabRatAFP did not develop tumors whereas 1/36 (3%) of the normal horse-serum treated animals did not develop tumors. The majority of those animals which were cured of their tumors by HabRatAFP treatment resisted tumor rechallenge. The mechanisms responsible for the anti-tumor effect of HabRatAFP were studied AH-66 tumor cells which were placed into millipore chambers, implanted i.p., were killed by the i.p. administration of HabRatAFP. Studies were conducted to see if treatment with HabRatAFP to rat AFP might increase the immunogenicity of an inoculum of X-irradiated tumor cells. In 1 experiment, no animals which received only X-irradiated tumor cells resisted tumor rechallenge with 5 .times. 106 tumor cells, whereas 6/11 (54.5%) of those animals which received X-irradiated tumor cells and treatment with HabRatAFP resisted tumor rechallenge.