Adenosine 3':5'-cyclic monophosphate as mediator of catabolite repression in Escherichia coli.
- 1 June 1975
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 72 (6) , 2300-2304
- https://doi.org/10.1073/pnas.72.6.2300
Abstract
Measurements of intracellular adenosine 3':5'-cyclic monophosphate (cAMP) concentrations in E. coli under a variety of conditions show that levels of this nucleotide are well correlated with the rate of synthesis of beta-galactosidase (beta-D-galactoside galactohydrolase, EC 3.2.1.23) in both catabolite repression and transient repression. These results, combined with extensive genetic and in vitro studies from a number of laboratories on the role of cAMP in E. coli, provide strong support for the concept that intracellular cAMP levels mediate the effects of catabolite and transient repression on rates on enzyme synthesis. Under all conditions studied, excretion can be described by a single rate constant, 2.1 min-1 at 37 degrees, indicating that intracellular levels cannot be regulated by alterations in the rate of cAMP excretion. Our data are fully consistent with the idea that carbon sources control intracellular cAMP levels by effects on its synthesis.Keywords
This publication has 13 references indexed in Scilit:
- Glucose Inhibition of Adenylate Cyclase in Intact Cells of Escherichia coli BProceedings of the National Academy of Sciences, 1974
- Cyclic 3′:5′-Adenosine Monosphate in Escherichia coli during Transient and Catabolite RepressionProceedings of the National Academy of Sciences, 1974
- Are cyclic AMP effects related to real physiological phenomena?Biochemical and Biophysical Research Communications, 1974
- Cyclic adenosine 3',5'-monophosphate in Escherichia coli.1973
- Cyclic Adenosine 3′,5′-Monophosphate-dependent Protein Kinase of Human Erythrocyte MembranesJournal of Biological Chemistry, 1972
- Electrophoretic analysis of the major polypeptides of the human erythrocyte membraneBiochemistry, 1971
- Cyclic Adenosine Monophosphate in BacteriaScience, 1970
- Cyclic AMP Receptor Protein of E. coli: Its Role in the Synthesis of Inducible EnzymesProceedings of the National Academy of Sciences, 1970
- Mechanism of Activation of Catabolite-Sensitive Genes: A Positive Control SystemProceedings of the National Academy of Sciences, 1970
- Pleiotropic deficiency of carbohydrate utilization in an adenyl cyclase deficient mutant of Escherichia coliBiochemical and Biophysical Research Communications, 1969