Preparation and Study of a Fluorescent Sugar Analog Competitive Inhibitor of Yeast Hexokinase
- 1 January 1979
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 9 (1) , 85-95
- https://doi.org/10.1080/00327487908061674
Abstract
The preparation of 2-deoxy-2-amino-N-(5-dimethylamino-l-naphthalene sulfonyl)-glucose (III) designed as a fluorescent competitive inhibitor of hexokinase was achieved after reacting 2-deoxy-2-aminoglucose and l-dimethylamino-5-naphthalene sulfonyl chloride. (III) showed fluorescence excitation and emission maxima in water at 330 and 507 nm, respectively. (III) was found to competitively inhibit hexokinase and a value of Ki = 3.0 × 10−3 M was obtained for the system hexokinase B + Mg. ATP + glucose at pH 8.4.This publication has 15 references indexed in Scilit:
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