Quantification of haemoglobin binding of 4,4′-methylenebis(2-chloroaniline)(MOCA) in rats
- 1 August 1990
- journal article
- research article
- Published by Springer Nature in Archives of Toxicology
- Vol. 64 (6) , 451-458
- https://doi.org/10.1007/bf01977626
Abstract
4,4′-Methylenebis(2-chloroaniline) (MOCA) is used as a curing agent in the production of polyurethane. MOCA is carcinogenic in experimental animals. Haemoglobin adducts have been proposed as dosimeters of aromatic amines for biological monitoring. A quantitative method to determine the adduct has now been worked out in female Wistar rats dosed per os with 3.82, 14.2 and 16.2 μmol/kg14C-ring labeled MOCA or 0.25 and 0.50 mmol/kg unlabeled MOCA. MOCA bound in decreasing amounts to DNA, RNA and proteins of the lung, liver and kidney. Fractions of 0.19% and 0.026% of the dose were bound to the blood proteins haemoglobin and albumin, respectively. MOCA released by hydrolysis from haemoglobin was determined by HPLC with electrochemical detection or by GC-MS. Albumin did not form any hydrolysable adducts with MOCA.This publication has 28 references indexed in Scilit:
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