Native‐type DHP‐sensitive calcium channel currents are produced by cloned rat aortic smooth muscle and cardiac α1 subunits expressed in Xenopus laevis ooeytes and are regulated by α2‐ and β‐subunits

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Abstract
Native tissue‐like L‐type voltage‐dependent calcium channels (L‐VDCC's) were expressed by in vitro transcribed cRNA injection of rat aorta or rabbit cardiac α1 subunit into Xenopus laevis oocytes. Co‐injection of VSM‐α1 with the cloned skeletal muscle β‐subunit (SK‐β) of the L‐type VDCC significantly increased the expressed peak current amplitude without significant changes in kinetics. Similar results were obtained by co‐injection of cardiac α1 (DSHT‐α1) the cloned skeletal α2‐subunit (SK‐α2) or with SK‐β. The oocytes co‐expressing cRNA's retained L‐type VDCC pharmacology.