INFLUENCE OF METHODOLOGY ON GLYCOSYLATED HEMOGLOBIN VALUES IN NIGERIAN SUBJECTS WITH SICKLE-CELL HEMOGLOBINOPATHY

  • 1 January 1984
    • journal article
    • research article
    • Vol. 14  (4) , 265-269
Abstract
Glycosylated Hb (GHb) levels in nondiabetic Nigerians (with Hb genotypes AA, AS and SS) were determined by short column chromatography and thiobarbituric acid colorimetry. Levels of GHb obtained by the microchromatographic method were significantly different from one another in the 3 subject groups. The group mean GHb level was highest in subjects with sickle cell anemia and lowest in subjects with sickle cell trait. Using the colorimetric method, the mean GHb level of the normal subjects with Hb genotype AA was not significantly different from that of subjects with Hb genotype AS or SS. Results of GHb determinations by microchromatography in subjects with sickle cell anemia or trait must be interpreted with caution. The colorimetric method, though more tedious, gives more reliable results and should be the method of choice in subjects with concurrent diabetes and sickle cell anemia or trait.