Protection of Sediment-Adsorbed Transforming DNA Against Enzymatic Inactivation
- 1 August 1983
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 46 (2) , 417-420
- https://doi.org/10.1128/aem.46.2.417-420.1983
Abstract
The action of DNase I on transforming DNA, both adsorbed to marine sediment and in solution, was investigated. DNase I reduced the transformation frequencies of free DNA more than of adsorbed DNA. Changes in salt concentration or pH did not have a significant influence on the DNA-sediment complex. Soil components other than organic materials and clay minerals can bind DNA and retard its enzymatic degradation.This publication has 10 references indexed in Scilit:
- A Study on the Mechanism of DNA Excretion fromP. aeruginosaKYU-1—Effect of Mitomycin C on Extracellular DNA Production—Agricultural and Biological Chemistry, 1981
- Interaction of marine sediment with DNA and DNA availability to nucleasesMarine Biology, 1981
- Generic Assignments, Strain Histories and Properties of Pure Cultures of CyanobacteriaMicrobiology, 1979
- Genetic exchange in Bacillus subtilis in soilMolecular Genetics and Genomics, 1978
- Ultraviolet inactivation and excision-repair in Bacillus subtilis I. Construction and characterization of a transformable eightfold auxotrophic strain and two ultraviolet-sensitive derivativesMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1972
- Adsorption of ribonucleic acid on bentoniteAnalytical Biochemistry, 1969
- Genetic transformation of Bacillussubtilis by extracellular DNABiochemical and Biophysical Research Communications, 1962
- A procedure for the isolation of deoxyribonucleic acid from micro-organismsJournal of Molecular Biology, 1961
- Appearance of Genetic Transforming Activity in Pneumococcal CulturesScience, 1960
- ADSORPTION OF MONONUCLEOTIDES, NUCLEIC ACIDS, AND NUCLEOPROTEINS BY CLAYSSoil Science, 1952