Mediation of glucocorticoid receptor function by the activation of latent transforming growth factor beta 1 in MG‐63 human osteosarcoma cells

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Abstract
We analyzed glucocortcoid receptor function using ligand binding assays, DNA band-shift analysis and trans-activation of the murine mammary tumor virus-thymidine kinase-chloramphenicol acetyltransferase reporter gene in transiently transfected MG-63 human osteosarcoma cells. Dexamethasone increased the distribution of MG-63 cells in the G1/G0 phase of the cell cycle, thus decreasing the rate of DNA synthesis and cell growth. Its effect on MG-63 cell growth was neutralized by RU486 and anti-transforming growth factor beta 1 (TGFβ1) antibody. In addition, (i) dexamethasone increased the levels of active TGFβ1 in MG-63-conditioned media without significantly altering the expression of TGFβ1 mRNA in MG-63 cells and (ii) TGFβ1 inhibited proliferation of MG-63 cells. Therefore, we conclude that glucocorticoid receptor function is mediated by the activation of latent-TGFβ1 in MG-63 osteosarcoma cells. © 1995 Wiley-Liss, Inc.

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