Characterization of acid and alkaline phosphatase activity in preparations of tubulin and microtubule‐associated proteins
- 10 January 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 151 (1) , 54-58
- https://doi.org/10.1016/0014-5793(83)80341-x
Abstract
Acid and alkaline phosphatase activity, determined by the hydrolysis of p‐nitrophenyl phosphate, was found in preparation of microtubules purified from bovine brain by temperature‐dependent assembly—disassembly and ion‐exchange chromatography. Phosphocellulose‐purified tubulin contained an associated acid phosphatase activity, stimulated by Mg2+ and Zn2+. Alkaline phosphatase activity with a pH optimum of 10.4 was measured in a fraction of microtubule‐associated proteins (MAPs). Kinetics and the effects of sodium fluoride, sodium tartrate, sulfhydryl‐blocking agents, EDTA and Zn2+ are reported.Keywords
This publication has 13 references indexed in Scilit:
- Multiple phosphorylation sites of microtubule‐associated protein (MAP2) observed at high ATP concentrationsFEBS Letters, 1981
- Structure and phosphorylation of microtubule-associated protein 2 (MAP 2).Proceedings of the National Academy of Sciences, 1980
- Metal analysis by energy dispersive X-ray fluorescence of bovine brain tubulin and microtubule-associated proteins prepared by phosphocellulose chromatographyBiochimica et Biophysica Acta (BBA) - Protein Structure, 1980
- Inhibition of microtubule assembly by phosphorylation of microtubule-associated proteinsBiochemistry, 1980
- Autophosphorylation of brain microtubule protein: Evidence for endogenous protein kinase/phosphoprotein phosphatase cycling and multiple phosphorylation of a microtubule associated proteinBiochemical and Biophysical Research Communications, 1980
- SOME CHARACTERISTICS OF ATPase ACTIVITY IN A BRAIN MICROTUBULE PROTEIN PREPARATIONJournal of Neurochemistry, 1979
- ACID AND ALKALINE PHOSPHATASES IN A BRAIN TUBULIN PREPARATIONJournal of Neurochemistry, 1979
- PROTEIN PHOSPHORYLATION IN VITRO IN BRAIN TUBULIN PREPARATIONS: EFFECTS OF Zn2+ AND CYCLIC NUCLEOTIDESJournal of Neurochemistry, 1977
- Induction of a sheet polymer of tubulin by Zn2+Experimental Cell Research, 1976
- A protein factor essential for microtubule assembly.Proceedings of the National Academy of Sciences, 1975