Multiple pathways of interferon-induced gene expression in murine macrophages

Publisher Website
Google Scholar
Abstract
Steady-state levels of mRNAs that encode specific FcyR and la antigen genes have been measured in macrophages treated with interferons (IFNs) to examine the induction of these markers at the molecular level. Our previous studies suggested requirement for protein kinase C (PKC) in the IFN induction of these macrophage surface markers, although a difference in PKC dependence was found between IFN-α/β- and IFN-γ-induced FcγR expression. The protein kinase antagonist H 7, used previously to distinguish between the surface induction of FcyR by IFN-α/β and IFN-γ, also distinguishes between the IFN-γ and IFN-γ in the induction of FcyRI mRNA and FcyRI surface expression. Protein kinase inhibitors blocked the IFN-γ induction of la mRNA in a manner similar to that reported previously for cell surface la expression. It is concluded that FcyRI is induced by both IFN-γ and IFN-γ through distinct biochemical pathways, whereas IFN-γ utilizes distinct pathways to induce the two macrophage activation markers, la antigen and FcyRI.

This publication has 0 references indexed in Scilit: