The Clearance and Degradation of Oligodeoxynucleotides Following Intravenous Injection into Rabbits
- 1 January 1991
- journal article
- research article
- Published by Mary Ann Liebert Inc in Antisense Research and Development
- Vol. 1 (2) , 153-160
- https://doi.org/10.1089/ard.1991.1.153
Abstract
A 32P-labeled oligodeoxynucleotide of 20 bases was found to be quite stable in freshly drawn rabbit or human blood and no degradation was apparent within 2 hr. In contrast, following injection into rabbits, the same oligodeoxynucleotide was cleared from the blood rapidly and at least 17% was completely degraded within 5 min. After 90 min, 16% of the oligonucleotide was found largely intact in the urine. At this time, radioisotope was also found in all the solid tissues tested but probably not as intact oligonucleotide.Keywords
This publication has 8 references indexed in Scilit:
- Specific regulation of gene expression by antisense, sense and antigene nucleic acidsBiochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1990
- Antisense oligonucleotides: a new therapeutic principleChemical Reviews, 1990
- Conjugates of oligonucleotides and modified oligonucleotides: a review of their synthesis and propertiesBioconjugate Chemistry, 1990
- High-performance liquid chromatographic analysis of phosphorothioate analogues of oligodeoxynucleotides in biological fluidsJournal of Chromatography B: Biomedical Sciences and Applications, 1990
- Oligodeoxynucleoside phosphoramidates and phosphorothioates as inhibitors of human immunodeficiency virus.Proceedings of the National Academy of Sciences, 1988
- Inhibition of human immunodeficiency virus replication by antisense oligodeoxynucleotides.Proceedings of the National Academy of Sciences, 1988
- Physicochemical properties of phospborothioate oligodeoxynucleotidesNucleic Acids Research, 1988
- DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mappingNucleic Acids Research, 1974