SLOW REACTING SUBSTANCE OF ANAPHYLAXIS (SRS-A) RELEASE FROM GUINEA-PIG LUNG PARENCHYMA DURING ANTIGEN-INDUCED OR IONOPHORE-INDUCED CONTRACTION

  • 1 January 1982
    • journal article
    • research article
    • Vol. 221  (1) , 146-151
Abstract
A dual isolated organ technique comprised of a guinea pig lung parenchymal strip and a guinea pig ileum was used to determine if slow reacting substance of anaphylaxis (SRS-A) is released from parenchyma during contractions evoked by antigen (ovalbumin) or by ionophore A23187 [calcimycin]. An immunologically sensitized parenchyma served as the primary target organ for ovalbumin and either a sensitized or unsensitized parenchyma was the target tissue for A23187; an unsensitized ileum functioned as the assay organ. In the presence of pyrilamine and indomethacin, ovalbumin or A23187 produced contractions of the parenchyma and concomitantly caused release of SRS-A from the lung strip which was indicated by a contraction of the ileum. The ileal response was antagonized by FPL 55712 [7-[3-(4-acetyl-3-hydroxy-2-propylphenoxy)-2-hydroxypropoxy]-4-oxo-8-propyl- 4H-1-benzopyran-2-carboxylic acid monosodium], whereas the parenchyma contractions were unaffected. Additional experiments were conducted in which parenchyma was contracted with histamine. At the height of the histamine contraction, the bathing fluid surrounding the parenchyma was removed and assayed on a pyrilamine-treated ileum. SRS-A was not detected, indicating that SRS-A release from parenchyma is not a function of tissue contraction, but is related to the antigen- and ionophore-induced contractions. To explain the lack of effect of FPL 55712 on parenchymal contractions to antigen or ionophore, the degree of antagonism produced by FPL 55712 on SRS-A contraction of parenchyma and ileum was compared. Evidently, at least 2 different classes of SRS-A receptors exist and those in the ileum and lung differ.