Characterization of pro-opiocortin, a precursor to opioid peptides and corticotropin.
- 1 February 1978
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 75 (2) , 669-671
- https://doi.org/10.1073/pnas.75.2.669
Abstract
The high MW (approximately 30,000) precursor to opioid activity (pro-opiocortin) previously detected in extracts of rat pituitary was digested with trypsin, and the resulting peptide mixture was resolved by high-performance reverse-phase chromatography. A peak of opioid activity was eluted at the position of the nonapeptide .beta.-LPH [.beta.-liptotropin] (61-69), which was also the same fragment obtained by trypsin digestion of .beta.s-lipotropin or .beta.-endorphin. This identified the protein as a precursor to the endorphins and Met-enkephalin. No activity was detected in the position corresponding to the Leu5 analog of .beta.s-LPH (61-69), thus ruling out the possibility of a .beta.-lipotropin-liek precursor to Leu-enkephalin in pituitary extracts. Proopiocortin and .beta.-lipotropin are present in rat pituitary extracts in comparable amounts, approximately 10 pmol/mg of tissue.This publication has 7 references indexed in Scilit:
- Isolation and characterization of the opioid peptides from rat pituitary: beta-endorphin.Proceedings of the National Academy of Sciences, 1977
- β-Endorphin and Adrenocorticotropin Are Selected Concomitantly by the Pituitary GlandScience, 1977
- Isolation and characterization of the opioid peptides from rat pituitary: beta-lipotropin.Proceedings of the National Academy of Sciences, 1977
- Common precursor to corticotropins and endorphinsProceedings of the National Academy of Sciences, 1977
- Purification and partial chemical characterization of human pituitary lipolytic hormoneCanadian Journal of Biochemistry, 1976
- Action of plasmin on ovine beta-lipotropin: Revision of the car☐yl terminal sequenceBiochemical and Biophysical Research Communications, 1973
- Isolation of bovine β-lipotropic hormoneBiochimica et Biophysica Acta (BBA) - Protein Structure, 1967