Absorption Spectra and Relative Photostability of the Different Forms of Chlorophyll in Chlorella.

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Abstract
When a suspension of broken Chlorella cells, clarified by centrifugation, is partially bleached by red light, the shape of the absorption curve changes and the peak position moves towards shorter wave lengths. This shift is attributed to differing photosensitivity of the different forms of chlorophyll which have different but overlapping absorption spectra in situ. The red peaks of the individual components appear to have the following peak positions and widths in m[mu] at half-height: chlorophyll b: 653(15); chlorophyll a: 673(18), 683(14), 695(15). The fraction of the total absorption due to each component and their relative sensitivity to bleaching by red light have been estimated.