Comparative Conformations of Uridine and Pseudouridine and Their Derivatives
- 1 July 1980
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 108 (2) , 457-463
- https://doi.org/10.1111/j.1432-1033.1980.tb04742.x
Abstract
Stimulated by the suggestion that pseudouridine Ψ‐39 in yeast tRNAPhe could be in a syn conformation [R. E. Hurd and B. R. Reid (1977) Nucleic Acid Research 4, 2747–2755], we have made a comparative study of the solution conformations of Ψ and U derivatives, using the proton‐proton Overhauser effect. Rotation around the glycosidic bond is observed for pseudouridine and 3′Ψ MP as well as for uridine and 3′UMP. However pseudouridine and 3′Ψ MP are mostly syn, whereas uridine, 3′UMP and 5′UMP are mostly anti. There is no evidence for a correlation between sugar conformation and orientation around the glycosidic bond. The results are confirmed by relaxation measurements. They are discussed in the light of earlier studies. They tend to support the suggestion of Hurd and Reid. They raise the question of the orientation of the pseudouridine found elsewhere in tRNA (e.g. in the TΨC loop).This publication has 41 references indexed in Scilit:
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