Purification and Properties of an Esterase B4 from Human Liver
- 1 January 1976
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 61 (2) , 331-335
- https://doi.org/10.1111/j.1432-1033.1976.tb10026.x
Abstract
A butyryl esterase, designated B4, has been purified from human liver and some of its properties described. The activity of this enzyme comprises 0.48% of the total butyryl esterase activity found in human liver. Esterase B4 has been distinguished from other butyryl esterases by its preference for the esters of the fluorogenic compounds 4-methyl umbilliferone and fluorescein over naphthyl esters as substrates. Other distinguishing features of this esterase include a relatively high pI (pH 8.7) A monomeric structure of low molecular weight (20 000) and high solubility in solutions of ammonium sulphate.Keywords
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