Functional heterogeneity of macrophage precursor cells from spleen of Leishmania donovani-infected and untreated mice.

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Abstract
We recently described the bone marrow-derived macrophage precursor, which is able to spontaneously and extracellularly kill protozoa of the genus Leishmania. These nonadherent, nonphagocytic macrophage precursor cells are present in the spleen of healthy mice only in a small quantity. However, high numbers of proliferating macrophage precursors are isolated from the spleen of Leishmania donovani-infected mice. Macrophage precursors from spleens of diseased animals are able to kill spontaneously the promastigote as well as the amastigote form of L. donovani. The mechanism of the spontaneous leishmanicidal activity of macrophage precursor cells derived from spleens of L. donovani-infected mice was investigated. This effector function could be defined in part as an antibody-dependent cellular cytotoxicity. In addition we assessed the role of CSF-1-containing L cell-conditioned supernatant at the leishmanicidal activity of these immature cells of the macrophage lineage. For that purpose, nonadherent spleen cells from healthy mice were cocultivated with this CSF-1-containing medium for 4 days. These in vitro proliferated macrophage precursor cells from untreated mice showed an increased leishmanicidal activity. Thereby we established a further activation mechanism for proliferating splenic macrophage precursor cells responsible for the observed killing of L. donovani pro- and amastigotes. The spontaneous cytotoxicity of macrophage precursors from spleens of L. donovani-diseased animals is thus defined as a cooperative effect of antibody-dependent cellular cytotoxicity and Macrophage-CSF activation.