Molecular epidemiology of Vibrio cholerae O1 isolates from Colombia
- 1 July 1997
- journal article
- Published by Microbiology Society in Journal of Medical Microbiology
- Vol. 46 (7) , 611-616
- https://doi.org/10.1099/00222615-46-7-611
Abstract
A total of 173 Vibrio cholerae O1 isolates from the recent cholera epidemic in Colombia was analysed by the polymerase chain reaction (PCR) for the genes encoding the A subunit of cholera toxin (ctx A) and the zonula occludens toxin (zot), and by ribotyping. All isolates were positive for ctx A and zot, which was confirmed by hybridisation. Ribotyping with restriction endonuclease Bg/I digestion of total DNA revealed three ribotypes: B5a comprising 165 (96.4%) isolates, and two new designated ribotypes B20 and B21a in six (3.5%) isolates and two (1.1%) isolates, respectively. These findings have significant public health implications.Keywords
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