The Stability of a Codon Transfer RNA Complex
Open Access
- 1 February 1970
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 12 (3) , 527-532
- https://doi.org/10.1111/j.1432-1033.1970.tb00882.x
Abstract
Equilibrium dialysis experiments indicate complex formation between the trinucleotide ApUpGp and tRNAfMet, the dissociation constant of the complex being approximately 150 μM. Unfractionated tRNA containing only minute quantities of tRNAMet fails to form complexes with ApUpGp to a detectable extent thus suggesting that the binding ability for this triplet is displayed solely by methionine accepting tRNAs.Deacylation of tRNA appears not to interfere with its complex forming ability. The strength of a trinucleotide—tRNA interaction is not altered measurably if the terminal phosphate group is detached from the triplets.The observed binding phenomenon is interpreted as a codon—anticodon interaction in the absence of ribosomes.Keywords
This publication has 23 references indexed in Scilit:
- Polypetide chain termination: An active processJournal of Molecular Biology, 1968
- Nachweis einer Wechselwirkung zwischen Trinucleotiden und löslichen RibonucleinsäurenHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1967
- The role of N-formyl-methionyl-sRNA in protein biosynthesisJournal of Molecular Biology, 1966
- Polynucleotide Synthesis and the Genetic CodeCold Spring Harbor Symposia on Quantitative Biology, 1966
- Studies on the Translation of the Genetic Message. I. Preparation of Oligonucleotide Messengers of Specified Base SequenceCold Spring Harbor Symposia on Quantitative Biology, 1966
- Conformational differences between s-RNA and aminoacyl s-RNABiochemical and Biophysical Research Communications, 1965
- Absorption and Optical Rotatory Dispersion of Seven Trinucleoside DiphosphatesJournal of Molecular Biology, 1965
- RNA Codewords and Protein SynthesisScience, 1964
- The Use of Esters of N-Hydroxysuccinimide in Peptide SynthesisJournal of the American Chemical Society, 1964
- A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counterAnalytical Biochemistry, 1960