Efficient gene transfer into mammalian primary endocrine cells with lipopolyamine-coated DNA.
- 1 September 1989
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 86 (18) , 6982-6986
- https://doi.org/10.1073/pnas.86.18.6982
Abstract
A general and efficient transfection procedure, based on compacted lipopolyamine-coated plasmids, has been developed. The active species is obtained by simple addition of excess synthetic lipospermine solution to the DNA and binds within minutes to the cell membrane. This technique has been developed on endocrine cells of the intermediate lobe of the pituitary as a general tool for physiological work on primary cells; it is not toxic and does not interfere with physiological regulations in melanotrope cells. A variety of eukaryotic cell cultures also have been transfected with success for transient and stable expression.Keywords
This publication has 19 references indexed in Scilit:
- Gene transfer from targeted liposomes to specific lymphoid cells by electroporation.Proceedings of the National Academy of Sciences, 1988
- Asbestos fibers mediate transformation of monkey cells by exogenous plasmid DNA.Proceedings of the National Academy of Sciences, 1988
- pH-sensitive immunoliposomes mediate target-cell-specific delivery and controlled expression of a foreign gene in mouse.Proceedings of the National Academy of Sciences, 1987
- Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure.Proceedings of the National Academy of Sciences, 1987
- [16] Liposomes as carriers for in vivo gene transfer and expressionPublished by Elsevier ,1987
- An estrogen-responsive element derived from the 5′ flanking region of the Xenopus vitellogenin A2 gene functions in transfected human cellsCell, 1986
- GABAA and GABAB receptors on porcine pars intermedia cells in primary culture: Functional role in modulating peptide releaseNeuroscience, 1986
- Equilibrium dialysis studies of polyamine binding to DNABiopolymers, 1982
- Liposomes: The Development of a New Carrier System for Introducing Nucleic Acids into Plant and Animal CellsPublished by Springer Nature ,1982
- A new technique for the assay of infectivity of human adenovirus 5 DNAVirology, 1973