The tissue content of cyclic AMP in rats after microwave irradiation in vivo.

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Abstract
When anesthetized rats were exposed to microwave (power output 1.3 kW, frequency 2450 MHz), the cAMP phosphodiesterase activity showed a rapid decline in the skeletal muscle, heart, trachea and lung; no activity essentially could be detected after a 30 s irradiation. cAMP levels in these tissues decreased rapidly to constant values within 30 s. Concentrations of cAMP in the heart, skeletal muscle, trachea and lung, measured by the tissue fixation with liquid N, were .apprx. 3, 3, 4 and 4 times higher than those after microwave irradiation, respectively. The i.v. injection of procaterol (1 .mu.mol/kg), a .beta.2-adrenoceptor agonist, caused a 6-fold increase in the tracheal cAMP level when measured after microwave fixation but only a 2-fold increase when measured after liquid N fixation. Rapid tissue fixation by microwave irradiation may provide a useful means for obtaining reliable values of the tissue cAMP content.