Effect of ventilation and pH on removal of mescaline and biogenic amines by rabbit lung

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Abstract
Perfused rabbit lungs, statically inflated with room air, removed 48% of perfused [14C]mescaline (10 .mu.M). When these lungs were ventilated for 1 min with room air, percentage mescaline removal increased to 76%. Ventilation with room air also produced small but statistically significant alterations of removal of 0.1 .mu.M 5-[14C]hydroxytryptamine and 0.1 .mu.M [14C]norepinephrine. This ventilatory effect was not dependent on amine oxidase, since lungs coperfused with an inhibitor of mescaline deamination still removed more perfused mescaline when ventilated with room air than when statically inflated. The effluent pH of lungs ventilated with room air rose to 8.25 .+-. 0.07 compared to 7.62 .+-. 0.06 during static inflation. Such pH alterations did not occur in lungs ventilated with 95% air-5% CO2, nor did mescaline removal during ventilation with this gas mixture differ from that during static inflation. When the pH of medium perfusing statically inflated lungs was increased from 7.3 to 7.9, removal of perfused mescaline (0.1 .mu.M) increased by 54%. Results from this study indicate that increased [14C]mescaline removal during ventilation with room air is associated with effluent pH alterations. In the absence of such pH changes, ventilation does not affect amine removal.