CHEMICAL FEATURES OF SPORE COAT OF BACILLUS SUBTILIS
- 1 January 1976
- journal article
- research article
- Published by Microbiology Research Foundation in The Journal of General and Applied Microbiology
- Vol. 22 (1) , 51-63
- https://doi.org/10.2323/jgam.22.51
Abstract
The spore coat of B. subtilis was isolated and solubilized by treatment with sodium dodecyl sulfate (SDS) plus dithiothreitol (DDT) solution. This treatment solubilized about 85% of the spore coat fraction and the soluble fraction mainly consisted of protein. The protein was purified in a buffer containing the detergent. The results of gel filtration, SDS-disc gel electrophoresis and NH2-terminal analysis showed the major component of the spore coat protein to be 1 kind with a MW of 14,000.This publication has 3 references indexed in Scilit:
- The NH2-terminal Residues of Bacillus subtilis ProteinsJournal of Biological Chemistry, 1968
- Biosynthesis of bacterial spore coatsJournal of Molecular Biology, 1968
- BEHAVIOR OF BACTERIAL SPORES IN AQUEOUS POLYMER TWO-PHASE SYSTEMSJournal of Bacteriology, 1961