Pituitary Adenylate Cyclase-Activating Peptide is a Potent Modulator of Human Colonic Motility
- 1 January 1993
- journal article
- Published by Taylor & Francis in Scandinavian Journal of Gastroenterology
- Vol. 28 (7) , 625-632
- https://doi.org/10.3109/00365529309096101
Abstract
Pituitary adenylate cyclase-activating peptide (PACAP) represents a novel brain-gut peptide with high sequence homology to vasoactive intestinal polypeptide (VIP). Since PACAP has been identified in the human gut, the effect of the two molecular forms PACAP-(1-38) and PACAP-(1-27), the hybrid PACAP-(1-23)VIP-(24-28), and VIP on the contractility of the longitudinal muscle of human sigmoid colon was tested in vitro. All peptides inhibited the spontaneous phasic contractions and relaxed concentration-dependently carbachol-precontracted preparations. The effects of the peptides remained unaffected by tetrodotoxin, by inhibition of phosphodiesterase activity, and by inhibition of nitric oxide synthesis. Apamin reduced only the effects of the PACAP peptides, whereas tetraethylammonium blocked only the effect of VIP. In conclusion, PACAP peptides and VIP mediate their relaxant effects via activation of specific PACAP and VIP receptors coupled to different potassium channels.Keywords
This publication has 27 references indexed in Scilit:
- Characterization of a guanosine‐nucleotide‐binding‐protein‐coupled receptor for pituitary adenylate‐cyclase‐activating polypeptide on plasma membranes from rat brainEuropean Journal of Biochemistry, 1991
- Tissue Distribution of PACAP as Determined by RIA: Highly Abundant in the Rat Brain and TestesEndocrinology, 1991
- Binding sites of a novel neuropeptide pituitary‐adenylate‐cyclase‐activating polypeptide in the rat brain and lungEuropean Journal of Biochemistry, 1990
- Molecular identification of receptor for pituitary adenylate cyclase activating polypeptideBiochemical and Biophysical Research Communications, 1990
- Isolation of a neuropeptide corresponding to the N-terminal 27 residues of the pituitary adenylate cyclase activating polypeptide with 38 residues (PACAP38)Biochemical and Biophysical Research Communications, 1990
- The novel VIP-like hypothalamic polypeptide PACAP interacts with high affinity receptors in the human neuroblastoma cell line NB-OKPeptides, 1990
- A novel peptide which stimulates adenylate cyclase: Molecular cloning and characterization of the ovine and human cDNAsBiochemical and Biophysical Research Communications, 1990
- Expression of Ca2+ Mobilizing Receptors in Xenopus Oocytes: A Tool for Receptor CharacterizationDigestion, 1990
- Isolation of a novel 38 residue-hypothalamic polypeptide which stimulates adenylate cyclase in pituitary cellsBiochemical and Biophysical Research Communications, 1989
- Excitatory effects of adrenaline upon isolated preparations of human colonGut, 1972