Determination Of Fatty Acids And Carbohydrate Monomers In Micro-Organisms By Means Of Glass Capillary Gas Chromatography: Analysis Of Mycobacterium gordonae and Mycobacterium scrofulaceum

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Abstract
Trifluoroacetylated whole-cell methanolysates of 4 strains each of M. gordonae and M. scrofulaceum were analyzed by gas chromatography, using a glass capillary column. The major chromatographic peaks were identified by mass spectrometry as derivatives of fatty acids and carbohydrates. In addition, 2 predominant peaks, present in chromatograms representing M. scrofulaceum, were identified as 2-octadecanol and 2-eicosanol. These secondary alcohols were not found in any of the strains of M. gordonae studied. The amount of tuberculostearic acid in the latter species was < 1% of that in M. scrofulaceum.