Synthetic peptides from four separate regions of the poliovirus type 1 capsid protein VP1 induce neutralizing antibodies.
- 1 February 1985
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 82 (3) , 910-914
- https://doi.org/10.1073/pnas.82.3.910
Abstract
Peptides from different regions of the poliovirus type I capsid protein VP1 were synthesized. Antibodies raised against these peptides in rabbits and rats recognized the cognate peptides and denatured VP1. Peptides from 4 regions of VP1 generated antisera with neutralizing titers specifically against poliovirus type 1. Antisera against all other regions of VP1 failed to neutralize virus infectivity, although some of the antisera clearly bound to native virions. Thus, the neutralizing determinants on VP1 reside in specific noncontiguous regions of the protein and can be defined by specific peptides from these regions.This publication has 30 references indexed in Scilit:
- Priming for and induction of anti-poliovirus neutralizing antibodies by synthetic peptidesNature, 1983
- Critical role of an eight-amino acid sequence of VP1 in neutralization of poliovirus type 3Nature, 1983
- Preliminary studies of crystals of poliovirus type IJournal of Molecular Biology, 1982
- Protection against foot-and-mouth disease by immunization with a chemically synthesized peptide predicted from the viral nucleotide sequenceNature, 1982
- Antibodies against the chemically synthesized genome-linked protein of poliovirus react with native virus-specific proteinsCell, 1982
- Primary structure, gene organization and polypeptide expression of poliovirus RNANature, 1981
- HUMAN β‐ENDORPHIN: SYNTHESIS AND CHARACTERIZATION OF ANALOGS IODINATED AND TRITIATED AT TRYOSINE RESIDUES 1 AND 27International Journal of Peptide and Protein Research, 1980
- Topographical Studies on Poliovirus Capsid Proteins by Chemical Modification and Cross-linking with Bifunctional ReagentsJournal of General Virology, 1979
- New procedures for preparation and isolation of conjugates of proteins and a synthetic copolymer of D-amino acids and immunochemical characterization of such conjugatesBiochemistry, 1979
- Chemical Synthesis of Peptides and ProteinsAnnual Review of Biochemistry, 1970